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3D bioprinting patient-specific bone models
3D bioprinting is a state of the art additive manufacturing technique that has revolutionized tissue engineering strategies. The goal of this project is to 3D bioprint and analyse a cell-laden bone tissue engineering scaffold.
Keywords: Bioengineering, 3D bioprinting, Bone, Scaffold, Cell Culture, Bioreactor
Efforts to overcome the limitations of animal models have led to the emergence of in vitro human bone models. Advances such as 3D bioprinting allow us to control scaffold shapes and cell distributions to fabricate patient-specific models in an effort to better understand and treat disease.
Efforts to overcome the limitations of animal models have led to the emergence of in vitro human bone models. Advances such as 3D bioprinting allow us to control scaffold shapes and cell distributions to fabricate patient-specific models in an effort to better understand and treat disease.
The goal of this project is to fabricate a 3D in vitro model of bone, which will serve as a novel platform to improve our understanding of rare bone diseases. You will contribute to a project in which we will 3D bioprint cell-laden scaffolds and subsequently culture them in bioreactors. Then, a combination of non-destructive time-lapsed micro-computed tomography (micro-CT), histology, immunohistochemistry and gene expression techniques will be used to investigate cellular morphology, differentiation, and mineralization in the 3D bioprinted scaffolds. This interdisciplinary project will give you the opportunity to gain hands on experience in 3D bioprinting, bioreactor culture and scaffold analysis using techniques such as confocal microscopy and micro-CT imaging. The scaffolds produced in this project aim to emulate the natural extracellular matrix of trabecular bone tissue and will form the backbone for future experiments. Experience in cell culture is recommended however, the nature of the project can be tailored to your skills and research interests.
The goal of this project is to fabricate a 3D in vitro model of bone, which will serve as a novel platform to improve our understanding of rare bone diseases. You will contribute to a project in which we will 3D bioprint cell-laden scaffolds and subsequently culture them in bioreactors. Then, a combination of non-destructive time-lapsed micro-computed tomography (micro-CT), histology, immunohistochemistry and gene expression techniques will be used to investigate cellular morphology, differentiation, and mineralization in the 3D bioprinted scaffolds. This interdisciplinary project will give you the opportunity to gain hands on experience in 3D bioprinting, bioreactor culture and scaffold analysis using techniques such as confocal microscopy and micro-CT imaging. The scaffolds produced in this project aim to emulate the natural extracellular matrix of trabecular bone tissue and will form the backbone for future experiments. Experience in cell culture is recommended however, the nature of the project can be tailored to your skills and research interests.
Anke de Leeuw (adeleeuw@ethz.ch), Institute for Biomechanics, ETH Zürich Office address: HCP 18.1, Professorship: Ralph Müller
Anke de Leeuw (adeleeuw@ethz.ch), Institute for Biomechanics, ETH Zürich Office address: HCP 18.1, Professorship: Ralph Müller