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Development of Pulsatile Flow Loop for Studies on Endothelial Cells
In this project the student gets the chance to design and build a novel test bench which allows the generation of accurately controllable pulsatile flows for in-vitro studies on endothelial cells.
Keywords: test bench, pulsatile flow, fluidics, flow chamber, endothelial cells, von Willebrand factor
Von Willebrand Factor (vWF) is a large protein that plays a key role in coagulation and wound closure. It is secreted by endothelial cells (EC) into the blood stream, a process that is influenced among other factors by the strain and shear stress acting on the ECs. Patients with unphysiological blood flow conditions (e.g. through circulatory support pumps) often suffer from disrupted levels of vWF. Current studies suggest that impaired secretion of vWF could play a role. A better description of the correlation between mechanical stimuli on ECs and the secretion of vWF would help to understand underlying mechanisms and allow for new treatments. A detailed study of these effects in vitro requires a flow driving system capable of reproducing complex pulsatile flow profiles.
**Project Goal**
In this project, a test bench is to be developed which allows the generation of accurately controllable pulsatile flows for in-vitro studies on endothelial cells. The envisioned system should enable both steady and transient flows, including complex physiological profiles.
The project presents the opportunity of experiencing and driving the complete development process, including the design of a suitable concept for the test bench, selection and assembly of the hardware and the implementation of needed control systems. Initial operations including tests with alive cells and validations of the system will provide a satisfying end goal.
**In this project, you will:**
- experience the complete development process of an advanced fluidic test bench including concept design, assembly and initial operation
- benefit from the collaboration with an interdisciplinary group
- gain insights into bio-engineering lab work
**You bring with you:**
- basic knowledge of fluid dynamics
- basic experience with control systems
- interest for experimental work
- the ability to work independently and reliably
- a systematic and methodical approach to problems
**References:**
[1] Conway, D. E., et al. (2009). Am. J. Physiol.-Heart Circul. Physiol., 298(2), H367–H374.
[2] Turner, N. A., et al. (2009). Blood, 114(24), 5102–5111.
Von Willebrand Factor (vWF) is a large protein that plays a key role in coagulation and wound closure. It is secreted by endothelial cells (EC) into the blood stream, a process that is influenced among other factors by the strain and shear stress acting on the ECs. Patients with unphysiological blood flow conditions (e.g. through circulatory support pumps) often suffer from disrupted levels of vWF. Current studies suggest that impaired secretion of vWF could play a role. A better description of the correlation between mechanical stimuli on ECs and the secretion of vWF would help to understand underlying mechanisms and allow for new treatments. A detailed study of these effects in vitro requires a flow driving system capable of reproducing complex pulsatile flow profiles.
**Project Goal**
In this project, a test bench is to be developed which allows the generation of accurately controllable pulsatile flows for in-vitro studies on endothelial cells. The envisioned system should enable both steady and transient flows, including complex physiological profiles.
The project presents the opportunity of experiencing and driving the complete development process, including the design of a suitable concept for the test bench, selection and assembly of the hardware and the implementation of needed control systems. Initial operations including tests with alive cells and validations of the system will provide a satisfying end goal.
**In this project, you will:**
- experience the complete development process of an advanced fluidic test bench including concept design, assembly and initial operation
- benefit from the collaboration with an interdisciplinary group
- gain insights into bio-engineering lab work
**You bring with you:**
- basic knowledge of fluid dynamics
- basic experience with control systems
- interest for experimental work
- the ability to work independently and reliably
- a systematic and methodical approach to problems
**References:**
[1] Conway, D. E., et al. (2009). Am. J. Physiol.-Heart Circul. Physiol., 298(2), H367–H374.
[2] Turner, N. A., et al. (2009). Blood, 114(24), 5102–5111.
Not specified
Jonas Abeken, jonas.abeken@uzh.ch, +41 44 635 50 53
Jonas Abeken, jonas.abeken@uzh.ch, +41 44 635 50 53