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Simultaneous Optical and Electrical Imaging of Neural Activity
The main goal of this project is to combine optical and electrical functional readouts on microelectronics-based microelectrode array, in order to study neural network in vitro.
Keywords: MEA, CMOS, GEVI, Voltage Imaging, Cell Culture, Microscopy, AAV, IFA
High-Density MicroElectrode Arrays (**HD-MEAs**) enable to study physiological activity in neural networks by recording extracellular electrical signals. In our lab, we developed a cutting-edge HD-MEA featuring 26’400 platinum electrodes at 17.5 µm pitch, which is particularly suited to study neural populations. An alternative to electrical functional readouts are Genetically Encoded Voltage Indicators (**GEVIs**), a technology that allows to readout neural membrane potential by fluorescence readout. The goal of this thesis is to combine and compare HD-MEA technology and human GEVIs in order to extract relevant electrophysiological features from neurons. The project is multidisciplinary and involves cell handling, experiments with HD-MEA, data analysis and cell imaging.
For more info read:
http://pubs.rsc.org/en/Content/ArticleLanding/2015/LC/C5LC00133A#!divAbstract
http://www.jneurosci.org/content/36/39/9977
High-Density MicroElectrode Arrays (**HD-MEAs**) enable to study physiological activity in neural networks by recording extracellular electrical signals. In our lab, we developed a cutting-edge HD-MEA featuring 26’400 platinum electrodes at 17.5 µm pitch, which is particularly suited to study neural populations. An alternative to electrical functional readouts are Genetically Encoded Voltage Indicators (**GEVIs**), a technology that allows to readout neural membrane potential by fluorescence readout. The goal of this thesis is to combine and compare HD-MEA technology and human GEVIs in order to extract relevant electrophysiological features from neurons. The project is multidisciplinary and involves cell handling, experiments with HD-MEA, data analysis and cell imaging.