Register now After registration you will be able to apply for this opportunity online.
This opportunity is not published. No applications will be accepted.
Protocol development for assays of bone and cartilage tissues from mouse knee joints
Abstract: Reproducibly extracting and measuring biomarkers from small structures (e.g. mouse knees) can be challenging. The goal of this work is to develop protocols for this challenge.
Glycosaminoglycans, collagen, DNA, and mineral content are just some of the markers used to understand biological tissue quality. In this work, we are looking to develop robust protocols for assaying bone and cartilage, and to extract biomarkers of interest for diseases like arthritis. This work will form a foundation for future research projects.
Protocol development includes:
- identification of biomarkers of interest
- investigation of available assays and measurement techniques
- identification and testing of potential solutions with mouse tissues
- evaluation of solutions for future use & development
No specific prerequisites are required; but preference will be made to students possessing biochemistry or lab skills.
Glycosaminoglycans, collagen, DNA, and mineral content are just some of the markers used to understand biological tissue quality. In this work, we are looking to develop robust protocols for assaying bone and cartilage, and to extract biomarkers of interest for diseases like arthritis. This work will form a foundation for future research projects. Protocol development includes: - identification of biomarkers of interest - investigation of available assays and measurement techniques - identification and testing of potential solutions with mouse tissues - evaluation of solutions for future use & development
No specific prerequisites are required; but preference will be made to students possessing biochemistry or lab skills.
- 10% literature review & project planning;
- 20% identification of methods;
- 60% data collection & processing,
- 10% report & presentation preparation
- 10% literature review & project planning; - 20% identification of methods; - 60% data collection & processing, - 10% report & presentation preparation
Dr Kathryn Stok, kstok@unimelb.edu.au / Department of Biomedical Engineering, The University of Melbourne
Dr Kathryn Stok, kstok@unimelb.edu.au / Department of Biomedical Engineering, The University of Melbourne