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Bone organoids: cell self-organization and cell-cell interactions
In vitro 3D bone organoids aim to bridge the gap existing with current in vitro models (often on a 2D or represented by a single cell type) and in vivo situations. By definition organoid means resembling an organ this implies: multiple tissue-specific cell types, recapitulation some specific functio
Organoids have been considered to be complex 3D cellular structures capable to self-renew and self-organize that display architectures and functionalities similar as the tissue of origin. Organoids aim at recapitulating of a large number of biological parameters such as cell-cell- interactions, organization of heterogeneous site-specific cells. Bone is a dynamic tissue that suffers a continuous process of bone formation and bone resorption. Bone is composed by bone forming cells (osteoblast, osteocytes and bone lining cells) and bone resorption cells (osteoclasts). Cell location and activity is tightly regulated. Osteoblasts originate from mesenchymal stem cells and are the bone cells responsible for the production and maintenance of the bone architecture. At a certain moment some of the osteoblasts stop synthesizing bone matrix and become embedded within bone. Osteoblast cells that remain entrapped into the bone matrix differentiate to osteocytes. Osteoblasts can also remain on bone surface differentiating to bone lining cells. Besides of bone forming cells, bone is also constituted by cells involved in bone resorption processes. Osteoclasts are multinucleated cells derived from hematopoietic stem cells and are formed by fusion of precursor monocytes. During bone remodeling or following a bone fracture, cells from the osteoblast lineage and as well immune cells, express macrophage stimulating colony factor (M-CSF) or RANKL that induce mononuclear cells to fuse and form osteoclasts. Osteoclasts are situated on the bone surface and are responsible for bone resorption.
Organoids have been considered to be complex 3D cellular structures capable to self-renew and self-organize that display architectures and functionalities similar as the tissue of origin. Organoids aim at recapitulating of a large number of biological parameters such as cell-cell- interactions, organization of heterogeneous site-specific cells. Bone is a dynamic tissue that suffers a continuous process of bone formation and bone resorption. Bone is composed by bone forming cells (osteoblast, osteocytes and bone lining cells) and bone resorption cells (osteoclasts). Cell location and activity is tightly regulated. Osteoblasts originate from mesenchymal stem cells and are the bone cells responsible for the production and maintenance of the bone architecture. At a certain moment some of the osteoblasts stop synthesizing bone matrix and become embedded within bone. Osteoblast cells that remain entrapped into the bone matrix differentiate to osteocytes. Osteoblasts can also remain on bone surface differentiating to bone lining cells. Besides of bone forming cells, bone is also constituted by cells involved in bone resorption processes. Osteoclasts are multinucleated cells derived from hematopoietic stem cells and are formed by fusion of precursor monocytes. During bone remodeling or following a bone fracture, cells from the osteoblast lineage and as well immune cells, express macrophage stimulating colony factor (M-CSF) or RANKL that induce mononuclear cells to fuse and form osteoclasts. Osteoclasts are situated on the bone surface and are responsible for bone resorption.
The overall aim of this project is to go one step further and to identify the different bone cell types and organizations within our bone organoids. For this purpose the student will work the establishment of different immunostaining to specifically identify the different cell populations and location present in our bone organoids. The student will get trained on immunostaining strategies and imaging techniques. Task: 70% laboratory work, 20% data analysis, 10% report and presentation.
The overall aim of this project is to go one step further and to identify the different bone cell types and organizations within our bone organoids. For this purpose the student will work the establishment of different immunostaining to specifically identify the different cell populations and location present in our bone organoids. The student will get trained on immunostaining strategies and imaging techniques. Task: 70% laboratory work, 20% data analysis, 10% report and presentation.
If you are interested please send an e-mail with a short description of your interests and laboratory experience to: marina.rubert@hest.ethz.ch
Our work on Tissue engineering and Regeneration: http://www.bone.ethz.ch/research/tissue-engineering-and-regenerative-medicine.html
Our lab: http://www.bone.ethz.ch/the-group.html
Professorship: Ralph Müller
If you are interested please send an e-mail with a short description of your interests and laboratory experience to: marina.rubert@hest.ethz.ch
Our work on Tissue engineering and Regeneration: http://www.bone.ethz.ch/research/tissue-engineering-and-regenerative-medicine.html Our lab: http://www.bone.ethz.ch/the-group.html