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Investigation of brain vasculature and proteinopathies in transgenic mouse models of Alzheimer's disease
Here we aim to investigate the relation between brain vasculature abnormality and amyloid/tau deposits in mouse models of Alzheimer's disease using novel high-resolution fluorescence microscopy.
The abnormal deposition of beta-amyloid proteins and neurofibrillary tangles in the brain is one of the major histopathological hallmarks of Alzheimer’s disease. Currently available intravital microscopy techniques for high-resolution plaque visualization commonly involve highly invasive procedures and are limited to a small field-of-view within the rodent brain. We recently devised a large-field multi-focal illumination (LMI) fluorescence microscopy method that provides a whole brain field-of-view and high spatial and temporal resolutions. We have employed LMI for in vivo transcranial detection of amyloid deposits at single plaque resolution (<20 mm) in mouse models.
Here we aim to further investigate the relation between brain vasculature abnormality and amyloid/tau deposits in three mouse lines (APP/PS1, arcAbeta, P301L) using novel imaging probes. This knowledge will be important for understanding of the disease mechanism and for developing biomarker for Alzheimer’s disease with translational potential.
The experiments of project include
- Immunohistochemical staining and confocal microscopy to map the regional distribution of amyloid-beta plaques tauopathy in mouse brain sections.
- ELISA/MSD assays and other biochemical analysis
- Thioflavin T assay to characterize the binding property of imaging probes
- Data analysis; Image processing, registration.
Data analysis (40%), experiment (40%), Writing and reading (20%); Weekly group meeting and journal club
The abnormal deposition of beta-amyloid proteins and neurofibrillary tangles in the brain is one of the major histopathological hallmarks of Alzheimer’s disease. Currently available intravital microscopy techniques for high-resolution plaque visualization commonly involve highly invasive procedures and are limited to a small field-of-view within the rodent brain. We recently devised a large-field multi-focal illumination (LMI) fluorescence microscopy method that provides a whole brain field-of-view and high spatial and temporal resolutions. We have employed LMI for in vivo transcranial detection of amyloid deposits at single plaque resolution (<20 mm) in mouse models.
Here we aim to further investigate the relation between brain vasculature abnormality and amyloid/tau deposits in three mouse lines (APP/PS1, arcAbeta, P301L) using novel imaging probes. This knowledge will be important for understanding of the disease mechanism and for developing biomarker for Alzheimer’s disease with translational potential.
The experiments of project include - Immunohistochemical staining and confocal microscopy to map the regional distribution of amyloid-beta plaques tauopathy in mouse brain sections. - ELISA/MSD assays and other biochemical analysis - Thioflavin T assay to characterize the binding property of imaging probes - Data analysis; Image processing, registration. Data analysis (40%), experiment (40%), Writing and reading (20%); Weekly group meeting and journal club
- To further develop novel in vivo LMI fluorescence microscopy platform for amyloid and tau imaging.
- To investigate the vasculature abnormality and the link with proteinopathy in mouse models of Alzheimer's disease
- To further develop novel in vivo LMI fluorescence microscopy platform for amyloid and tau imaging. - To investigate the vasculature abnormality and the link with proteinopathy in mouse models of Alzheimer's disease
Dr.Ruiqing Ni, Institute for biomedical engineering, ETH Zurich & University of Zurich, Wolfgang-Pauli strasse 27, HIT E22.4.
ni@biomed.ee.ethz.ch
Dr.Ruiqing Ni, Institute for biomedical engineering, ETH Zurich & University of Zurich, Wolfgang-Pauli strasse 27, HIT E22.4. ni@biomed.ee.ethz.ch
Each year the IDEA League offers the students of its partner universities over 180 monthly grants for a short-term research exchange. In general, these grants are awarded based on academic merit. For more information visit http://idealeague.org/student-grant/