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Single-Cell Epitope mapping using Antigen-libraries and single-cell secretion assays
This project uses droplet based microfluidics to encapsulate single-cells for antibody capture, characterization and B cell isolation. The current project involves cloning and expression of novel antigen into an antigen-library for antigen mapping at single-droplet resolution. The project is suited for Master projects and master thesis (>3 months) and involves, cloning, validation, expression analysis as well as single-droplet verification. Finally, antibody secretion experiment are planned to isolate single-antigen binders and B cell sequencing.
Current antigens revolve around the SARS Cov2 Nucleocapsid protein, RBD and envelope proteins to assess antibody cross-reactivity.
Keywords: Single Cell Assays, Droplet microfluidics, B-cell Immunology, Molecular Biology, Antibody repertoires, B cell anergy
Overall, this project aim to optimize approaches to encapsulate, sort and validate antigen specificity from tumor-specific antibody secreting cells
Overall, this project aim to optimize approaches to encapsulate, sort and validate antigen specificity from tumor-specific antibody secreting cells
Ability to randomly encapsulate and enrich for tumor-specific cells
Ability to randomly encapsulate and enrich for tumor-specific cells
