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Novel Bioink Synthesis for 3D Bioprinting
3D bioprinting is a state of the art additive manufacturing technique that has revolutionized tissue engineering strategies. The goal of this project is to design a bioink, compatible with bioprinting able to support the formation of cell secreted collagen.
Bioinks are specialized hydrogels designed to enable 3D printing of microscale scaffolds. Bioinks are made by combining living cells and biocompatible polymers to mimic the extracellular matrix of tissues. These cell-encapsulating hydrogels are loaded into an extrusion-based system to create a biomimetic dynamic microenvironment providing a novel framework to model disease and study pharmacological therapies. However, bioinks are temperature sensitive, each ink is associated with a specific gelation temperature above which it is viscous yet printable and below which it solidifies. During this project, you will design a custom made bioink able to support the growth of human cells in order to print cellular constructs for bone models. Your designed bioink should be suitable for extrusion bioprinting, non-toxic, biocompatible and biodegradable. Furthermore, in the final construct we aim to study cell-secreted collagen thus your bioink should not interfere with collagen visualization. You may consider using classical natural materials such as alginate and gelatin or developing a modified synthetic ink with RGD sequences. The bioink will require shear thinning properties, rapid gelation and shape retention.
Bioinks are specialized hydrogels designed to enable 3D printing of microscale scaffolds. Bioinks are made by combining living cells and biocompatible polymers to mimic the extracellular matrix of tissues. These cell-encapsulating hydrogels are loaded into an extrusion-based system to create a biomimetic dynamic microenvironment providing a novel framework to model disease and study pharmacological therapies. However, bioinks are temperature sensitive, each ink is associated with a specific gelation temperature above which it is viscous yet printable and below which it solidifies. During this project, you will design a custom made bioink able to support the growth of human cells in order to print cellular constructs for bone models. Your designed bioink should be suitable for extrusion bioprinting, non-toxic, biocompatible and biodegradable. Furthermore, in the final construct we aim to study cell-secreted collagen thus your bioink should not interfere with collagen visualization. You may consider using classical natural materials such as alginate and gelatin or developing a modified synthetic ink with RGD sequences. The bioink will require shear thinning properties, rapid gelation and shape retention.
The goal of this project is to design a bioink able to support cell adhesion, differentiation and metabolic functions by emulating the natural extracellular matrix of bone tissue. The project will likely involve the design, synthesis and characterization of a hydrogel biomaterial however, the nature of the project may be subject to changes.
The goal of this project is to design a bioink able to support cell adhesion, differentiation and metabolic functions by emulating the natural extracellular matrix of bone tissue. The project will likely involve the design, synthesis and characterization of a hydrogel biomaterial however, the nature of the project may be subject to changes.
Anke de Leeuw (adeleeuw@ethz.ch), Institute for Biomechanics, ETH Zürich Office address: HCP 18.1, Professorship: Ralph Müller
Anke de Leeuw (adeleeuw@ethz.ch), Institute for Biomechanics, ETH Zürich Office address: HCP 18.1, Professorship: Ralph Müller