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Electrophysiological analysis of stretch activated ion channels under microgravity conditions.
Overexpression of specific stretch activated ion channels in Xenopus laevis oocytes, by DNA microinjection.
Subsequent characterization of the channels by electrophysiological methods under normal gravity and microgravity
Keywords: microgravity, mechanobiology, electrophysiology, two electrode voltage clamp, cation channel, Xenopus oocytes
We will overexpress specific ion channels in Xenopus laevis oocytes, by DNA microinjection. Subsequently we will characterize the channels by electrophysiological methods. The work of this project includes:
- preparing solutions and maintaining of Xenopus oocytes • optionally microinjection of RNA
- determine overexpression with qPCR and western blotting • use of the two electrode voltage clamp (TEVC)
- establishing of protocols to characterize overexpressed channels
- data analysis
- transfer established protocol to custom made “OoClamp” device
- fly experiment during a European Space Agency (ESA) parabolic flight campaign, if flight spot is confirmed.
We will overexpress specific ion channels in Xenopus laevis oocytes, by DNA microinjection. Subsequently we will characterize the channels by electrophysiological methods. The work of this project includes:
- preparing solutions and maintaining of Xenopus oocytes • optionally microinjection of RNA - determine overexpression with qPCR and western blotting • use of the two electrode voltage clamp (TEVC) - establishing of protocols to characterize overexpressed channels - data analysis - transfer established protocol to custom made “OoClamp” device - fly experiment during a European Space Agency (ESA) parabolic flight campaign, if flight spot is confirmed.
Analyze and describe the activation of transient receptor potential cation channels under microgravity conditions.
Analyze and describe the activation of transient receptor potential cation channels under microgravity conditions.
Fabian Ille,
Dr. sc. nat. ETH
fabian.ille@hslu.ch
Institute of Medical Engineering
Lucerne University of Applied Sciences and Arts
Lucerne School of Engineering and Architecture
Technikumstrasse 21, CH-6048 Horw
T direct +41 41 349 36 15
Office: Seestrasse 41, CH-6052 Hergiswil
Fabian Ille, Dr. sc. nat. ETH
fabian.ille@hslu.ch
Institute of Medical Engineering
Lucerne University of Applied Sciences and Arts Lucerne School of Engineering and Architecture