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Master Student Position in T cell development and negative selection
We are looking for a highly motivated master student with a strong interest in immunology, T cell development and autoimmune diseases.
Keywords: Immunology, T cell development, negative selection, autoimmune diseases
We are looking for a highly motivated master student with a strong interest in immunology, T cell development and autoimmune diseases. You will be working in a young and highly active multinational team. At the Institute of Laboratory Animal Science we are combining extensive experience in the fields of genetic modification to establish new animal models that provide an elementary tool to investigate T cell development and autoimmune diseases. You should have a completed Bachelor degree in Life Sciences, Immunology or similar, a strong interest in immunology and a high level of motivation. In addition, you should feel confident when performing standard molecular lab work. Experience with flow cytometry is of advantage but not required.
We are looking for a highly motivated master student with a strong interest in immunology, T cell development and autoimmune diseases. You will be working in a young and highly active multinational team. At the Institute of Laboratory Animal Science we are combining extensive experience in the fields of genetic modification to establish new animal models that provide an elementary tool to investigate T cell development and autoimmune diseases. You should have a completed Bachelor degree in Life Sciences, Immunology or similar, a strong interest in immunology and a high level of motivation. In addition, you should feel confident when performing standard molecular lab work. Experience with flow cytometry is of advantage but not required.
Hematopoietic precursor cells migrate from the bone marrow in the thymus where the T cell development is located. Thymocytes undergo several important steps during their development towards mature T cells. Because of the random nature of the TCR rearrangement the positive selection checkpoint is necessary to ensure that the double positive (DP) cells can interact with the MHC molecule. Negative selection, which is important to eliminate self-reactive thymocytes, is controlled by the avidity of the interaction between the TCR and the self-antigen. This means that cells which interact with a low avidity to self-antigen can survive and those with a high avidity die by apoptosis (negative selection). However, the exact molecular pathway of negative selection is not yet known. Therefore, we are analysing the role of specific transcription factors in negative selection and T cell development in different mouse strains. You will learn how to use state-of-the-art techniques like CRISPR/Cas9, flow cytometry, RT-PCR, western blot and others to analyze the T cell development in mice.
Hematopoietic precursor cells migrate from the bone marrow in the thymus where the T cell development is located. Thymocytes undergo several important steps during their development towards mature T cells. Because of the random nature of the TCR rearrangement the positive selection checkpoint is necessary to ensure that the double positive (DP) cells can interact with the MHC molecule. Negative selection, which is important to eliminate self-reactive thymocytes, is controlled by the avidity of the interaction between the TCR and the self-antigen. This means that cells which interact with a low avidity to self-antigen can survive and those with a high avidity die by apoptosis (negative selection). However, the exact molecular pathway of negative selection is not yet known. Therefore, we are analysing the role of specific transcription factors in negative selection and T cell development in different mouse strains. You will learn how to use state-of-the-art techniques like CRISPR/Cas9, flow cytometry, RT-PCR, western blot and others to analyze the T cell development in mice.
Please send your CV and a short motivation letter to: Jane Beil-Wagner, jane.beil-wagner@uzh.ch
Please send your CV and a short motivation letter to: Jane Beil-Wagner, jane.beil-wagner@uzh.ch