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Ex vivo evaulation of wound healing using granular biomaterials
Chronic wound care is hindered by the complex and variable proteomic profiles of wound exudates, which limit the efficacy of existing therapies. We aim to validate the effectiveness of our granular hydrogel platform in restoring balance to the wound microenvironment. Utilizing exudates obtained from diabetic foot ulcer (DFU) patients, we will optimize our microgel library to target clinically relevant cytokine profiles.
Supervisors: Apoorv Singh, BRIDGE fellow and Dr Börte Emiroglu, PIONEER fellow
Required experience: prior lab experience is desired, in particular in protein assays or cell assays.
Supervisors: Apoorv Singh, BRIDGE fellow and Dr Börte Emiroglu, PIONEER fellow
Required experience: prior lab experience is desired, in particular in protein assays or cell assays.
Initial studies will focus on preserving the performance established in vitro, followed by expanding the microgel library based on multiplex assays and proteomic data. Additionally, we will evaluate biomolecule diffusion, as well as fibroblast and endothelial cell migration/activation in response to treated exudates, with the goal of demonstrating enhanced tissue regeneration ex vivo.
Exudate Characterization: Multiplex cytokine assays and mass spectrometry-based proteomics will profile inflammatory mediators in DFU patient samples.
Microgel functionalization: Based on the cytokine profile obtained, key markers will be chosen and a library of microgels will be generated for combination into one multifunctional biomaterial.
Cell-based assays: Cell activation/migration assays – scratch assays (IBDI), IF staining, qPCR, WB.
Initial studies will focus on preserving the performance established in vitro, followed by expanding the microgel library based on multiplex assays and proteomic data. Additionally, we will evaluate biomolecule diffusion, as well as fibroblast and endothelial cell migration/activation in response to treated exudates, with the goal of demonstrating enhanced tissue regeneration ex vivo.
Exudate Characterization: Multiplex cytokine assays and mass spectrometry-based proteomics will profile inflammatory mediators in DFU patient samples.
Microgel functionalization: Based on the cytokine profile obtained, key markers will be chosen and a library of microgels will be generated for combination into one multifunctional biomaterial.
If working on something that could one day help improve patients’ lives is something you’re passionate about, write to us at boerte.emiroglu@chem.ethz.ch and apsingh@ethz.ch. Please include a CV, short cover letter/description including what experience you have, what you are looking to explore/learn through the project, and any other ideas you have that could be relevant. We look forward to hearing from you!
If working on something that could one day help improve patients’ lives is something you’re passionate about, write to us at boerte.emiroglu@chem.ethz.ch and apsingh@ethz.ch. Please include a CV, short cover letter/description including what experience you have, what you are looking to explore/learn through the project, and any other ideas you have that could be relevant. We look forward to hearing from you!
