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Investigation of brain vasculature and proteinopathies in transgenic mouse models of Alzheimer's disease
We aim to investigate the relation between brain vasculature abnormality and amyloid/tau deposits in three mouse lines (APP/PS1, arcAbeta, P301L) using novel imaging probes and cutting-edge optoacoustic imaging system.
The abnormal deposition of beta-amyloid proteins and neurofibrillary tangles in the brain is one of the major histopathological hallmarks of Alzheimer’s disease. Currently available intravital microscopy techniques for high-resolution plaque visualization commonly involve highly invasive procedures and are limited to a small field-of-view within the rodent brain. We recently devised a volumetric optoacoustic tomography (vMSOT) method that provides a whole brain imaging with high spatial and temporal resolutions. We have employed vMSOT for in vivo transcranial detection of amyloid deposits in mouse models.
Here we aim to further investigate the relation between brain vasculature abnormality and amyloid/tau deposits in three mouse lines (APP/PS1, arcAbeta, P301L) using novel imaging probes and cutting-edge imaging system. This knowledge will be important for understanding of the disease mechanism and for developing biomarker for Alzheimer’s disease with translational potential.
The experiments of project include
- Immunohistochemical staining and confocal microscopy to map the regional distribution of amyloid-beta plaques tauopathy in mouse brain sections.
- In vivo imaging in mouse models
- ELISA/MSD assays and other biochemical analysis
- Thioflavin T assay to characterize the binding property of imaging probes
- Data analysis; Image processing, registration.
-Light-sheet microscopy and brain clarity method.
Supervisor: Prof. Daniel Razansky; Dr Ruiqing Ni
The abnormal deposition of beta-amyloid proteins and neurofibrillary tangles in the brain is one of the major histopathological hallmarks of Alzheimer’s disease. Currently available intravital microscopy techniques for high-resolution plaque visualization commonly involve highly invasive procedures and are limited to a small field-of-view within the rodent brain. We recently devised a volumetric optoacoustic tomography (vMSOT) method that provides a whole brain imaging with high spatial and temporal resolutions. We have employed vMSOT for in vivo transcranial detection of amyloid deposits in mouse models.
Here we aim to further investigate the relation between brain vasculature abnormality and amyloid/tau deposits in three mouse lines (APP/PS1, arcAbeta, P301L) using novel imaging probes and cutting-edge imaging system. This knowledge will be important for understanding of the disease mechanism and for developing biomarker for Alzheimer’s disease with translational potential. The experiments of project include - Immunohistochemical staining and confocal microscopy to map the regional distribution of amyloid-beta plaques tauopathy in mouse brain sections. - In vivo imaging in mouse models - ELISA/MSD assays and other biochemical analysis - Thioflavin T assay to characterize the binding property of imaging probes - Data analysis; Image processing, registration. -Light-sheet microscopy and brain clarity method. Supervisor: Prof. Daniel Razansky; Dr Ruiqing Ni
to investigate the relation between brain vasculature abnormality and amyloid/tau deposits in three mouse lines (APP/PS1, arcAbeta, P301L) using novel imaging probes and cutting-edge optoacoustic imaging system.
to investigate the relation between brain vasculature abnormality and amyloid/tau deposits in three mouse lines (APP/PS1, arcAbeta, P301L) using novel imaging probes and cutting-edge optoacoustic imaging system.