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Semester Project / Master Thesis: Priming of mesenchymal stem cells for immunomodulatory cell therapy
Mesenchymal stem cells (MSCs) have an exceptional clinical potential, with possible applications in various inflammatory and degenerative diseases such as in osteoarthritis or disc degeneration. Preconditioning of MSCs (also known as priming) is a commonly used method to enhance the efficacy of MSCs. Various priming strategies exist, including pro-inflammatory priming and 3D culture priming. Little is known about how well MSCs can preserve this priming effect over time.
Keywords: MSC, stem cell, cell therapy, pro-inflammatory priming, 3D culture priming, spheroids, immunomodulation, RT-qPCR, cell culture, western blot, ELISA
In this project you will utilize pro-inflammatory cytokines and 3D cell culture to prime MSCs. You will investigate how the priming effect changes in various physiological inflammatory conditions over time. This gives us insights into how primed MSCs might behave after in vivo administration. During this project you will work with human mesenchymal stem cells and use various molecular biology methods including cell culture, RT-qPCR, ELISA, and possibly western blot.
In this project you will utilize pro-inflammatory cytokines and 3D cell culture to prime MSCs. You will investigate how the priming effect changes in various physiological inflammatory conditions over time. This gives us insights into how primed MSCs might behave after in vivo administration. During this project you will work with human mesenchymal stem cells and use various molecular biology methods including cell culture, RT-qPCR, ELISA, and possibly western blot.
The aim of this project is to investigate the temporal response of MSCs to pro-inflammatory and 3D culture priming.
The aim of this project is to investigate the temporal response of MSCs to pro-inflammatory and 3D culture priming.