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Master thesis: Preservation of stem cell spheroids
Cells grown in spheroids are an emerging technology with a tremendous translational potential in mesenchymal cell therapies. Preservation of cell spheroids is a critical step in developing spheroid-based cell therapeutics. We aim to compare different techniques to preserve MSC spheroids.
Standard 2D cell culture mimics very poorly the native cell environment, cell characteristics, and cell behavior is substantially altered. Cell spheroids are controlled aggregates of cells and represent a simple yet elegant way to mimic the native 3D environment. Cell spheroids preserve the native cell characteristics and behavior, which is an essential advantage for cell therapies.
A major challenge of the emerging spheroid technology is the storage and long-term culture of spheroids.
In this project we will compare and optimize techniques for MSC spheroid preservation. Different preservation techniques have different effects on cell viability and behavior. We will compare the impact of different preservation techniques (cryopreservation, alginate beads, ...) on viability and metabolic activity by means of established protocols (e.g. multi-dimensional flow cytometry). The goal is to optimize and validate an optimal preservation method for mesenchymal stem cell spheroids with respect to cell viability and cell behavior.
Tasks: 60% lab work, 20% method development, 20% documentation Requirements: Lab and basic cell culture experience are desired. Project start: Earliest start in June 2021
Location: The project will be done at the Balgrist Campus.
Standard 2D cell culture mimics very poorly the native cell environment, cell characteristics, and cell behavior is substantially altered. Cell spheroids are controlled aggregates of cells and represent a simple yet elegant way to mimic the native 3D environment. Cell spheroids preserve the native cell characteristics and behavior, which is an essential advantage for cell therapies. A major challenge of the emerging spheroid technology is the storage and long-term culture of spheroids. In this project we will compare and optimize techniques for MSC spheroid preservation. Different preservation techniques have different effects on cell viability and behavior. We will compare the impact of different preservation techniques (cryopreservation, alginate beads, ...) on viability and metabolic activity by means of established protocols (e.g. multi-dimensional flow cytometry). The goal is to optimize and validate an optimal preservation method for mesenchymal stem cell spheroids with respect to cell viability and cell behavior. Tasks: 60% lab work, 20% method development, 20% documentation Requirements: Lab and basic cell culture experience are desired. Project start: Earliest start in June 2021 Location: The project will be done at the Balgrist Campus.
The goal is to optimize and validate an optimal preservation method for mesenchymal stem cell spheroids with respect to cell viability and cell behavior.
The goal is to optimize and validate an optimal preservation method for mesenchymal stem cell spheroids with respect to cell viability and cell behavior.
Nick Herger: nick.herger@usz.ch
Stefan Dudli: stefan.dudli@usz.ch
Oliver Distler: oliver.distler@usz.ch
Nick Herger: nick.herger@usz.ch Stefan Dudli: stefan.dudli@usz.ch Oliver Distler: oliver.distler@usz.ch