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Phenotyping and Histological Confirmation of Fluorescent Reporter Mouse Model for Osteoblasts
Osteoblasts that encode specific endogenous proteins fused to a fluorescent reporter protein can be rapidly created through CRISPR/Cas9 genome editing.This, molecularly characterized fluorescently tagged osteoblasts will be used to study bone mechanomics
Keywords: confocal microscopy, immuohistochemistry, crispr, bone cells, mouse models
Refined in vivo models are needed to better understand mechano-molecular mechanisms crucial for bone adaptation and regeneration. We employed CRISPR/Cas9 genome editing technology to generate fluorescent reporter mice for studying in vivo single cell mechanomics of bone cells. This fluorescent reporter can be used for molecular characterization of osteoblasts in order to study in vivo single cell mechanomics of bone adaptation and regeneration. In order to validate the osteoblast specific mouse model, histological confirmation via target-specific immunostainings is required. In future projects, the fluorescent reporter mice will allow us to understand how osteoblasts behave and respond to mechanical signals in their local in vivo environment.
Refined in vivo models are needed to better understand mechano-molecular mechanisms crucial for bone adaptation and regeneration. We employed CRISPR/Cas9 genome editing technology to generate fluorescent reporter mice for studying in vivo single cell mechanomics of bone cells. This fluorescent reporter can be used for molecular characterization of osteoblasts in order to study in vivo single cell mechanomics of bone adaptation and regeneration. In order to validate the osteoblast specific mouse model, histological confirmation via target-specific immunostainings is required. In future projects, the fluorescent reporter mice will allow us to understand how osteoblasts behave and respond to mechanical signals in their local in vivo environment.
The aim of this project is to establish and apply both antibody based immunostainings and histological stainings to identify osteoblasts on mouse bone sections in fluorescent reporter mouse model.
The aim of this project is to establish and apply both antibody based immunostainings and histological stainings to identify osteoblasts on mouse bone sections in fluorescent reporter mouse model.
Dilara Yilmaz
Email: dilara.yilmaz@hest.ethz.ch, Institute for Biomechanics, ETH Zurich, Professorship Ralph Muller
Dilara Yilmaz Email: dilara.yilmaz@hest.ethz.ch, Institute for Biomechanics, ETH Zurich, Professorship Ralph Muller