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Characterization and investigating 2D skin disease model via biosensing and optical imaging
Pemphigus vulgaris (PV) is a unique group of autoimmune diseases. Researches have demonstrated that antibody-induced disruption of Dsg3 transadhesion initiates a signaling response in basal keratinocytes followed by loss of tissue integrity. The complexity of morphogenesis and tissue regeneration implies the existence of a transcellular communication network in which individual cells sense the environment and coordinate their biological activity in time and space.
To understand the fascinating ability of tissue self-organization, comprehensive study of biophysical properties (cell topography and bioelectricity) in combination with the analysis of biochemical networks (signaling pathways and genetic circuits) is required.
Together with the University of Bern and University of Lübeck, we aim to utilize the tools to study the topography and electrophysiology (cell potential, ion channel recording, localized ion detection, charges) of HPEK cells (human primary keratinocytes cells) to unravel the signaling pathways of the disease. We utilize optical imaging (fluorescence dyes) and biosensing tools (including the state of the art hs-SICM and electrical FluidFM setup) to study HPEK cells upon desmosome disruption.
In this project, you will have opportunity to carry out cell culture, gain expertise in wet-lab practices, work with the advanced biosensors and the exisiting setups in house, and apply the data analysis to study the signalling pathways of PV disease, in a supportive and thriving atmosphere. The project will and can be tailored to individual expertise and preference. You can choose a small topic among the overall big project.
If you are interested, please just send me an email (xumen@ethz.ch) to briefly introduce your background and motivation.
In this project, you will have opportunity to carry out cell culture, gain expertise in wet-lab practices, work with the advanced biosensors and the exisiting setups in house, and apply the data analysis to study the signalling pathways of PV disease, in a supportive and thriving atmosphere. The project will and can be tailored to individual expertise and preference. You can choose a small topic among the overall big project.
If you are interested, please just send me an email (xumen@ethz.ch) to briefly introduce your background and motivation.
To Investigating cells topography and eletrophysiology of HPEK cells
To Investigating cells topography and eletrophysiology of HPEK cells
Laboratory of Biosensors and Bioelectronics (LBB) Mengjia Xu xumen@ethz.ch
Laboratory of Biosensors and Bioelectronics (LBB) Mengjia Xu xumen@ethz.ch