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3D bioprinting of cell-laden mineralized silk fibroin biomimetic scaffold for bone tissue engineering
Natural bone is a living and complex tissue with various types of cells and nanohybrid organic-inorganic components. In this project, we try to fabricate cell-laden biomineralized silk fibroin composite scaffolds to mimic natural bone tissue using 3D bioprinting technique.
Keywords: 3D bioprinting, cell-laden, silk fibroin, organic-inorganic nanohybrid
Bone injuries and defects are a serious and costly health issue, and bone tissue engineering is one possible approach that aims at restoring the function of these damaged bone tissues with functional grafts. The next generation of engineering bone tissue aims at mimicking physiological bone tissue component and function by generating more complex and structurally organized implants. Natural bone is a living and complex tissue with various types of cells and nanohybrid organic-inorganic components. Bone cells (including osteoblasts, osteoclasts, osteocytes and mesenchymal stem cells) have a remarkable capacity for bone growth, regeneration, and remodeling. Bone extracellular matrix (ECM) is formed by a series of complex events involving mineralization with calcium phosphate in the form of hydroxyapatite (HAP) on ECM proteins primarily consisting of collagen type I. In order to mimic the natural bone tissue, two serious obstacles should be overcome. One is how to fabricate biomimetic bone scaffolds with living cells and maintain cell functions; the other one is how to mimic natural ECM with nanohybrid organic-inorganic components.
Recent advances in 3D bioprinting provide a promising approach to produce cell-laden biomimetic scaffolds. 3D bioprinting allows precise placement of cells, biochemical factors and biomaterial in a layer-by-layer process with personalized implant design. For mimicking the natural ECM, silk fibroin (SF) is one of the ideal candidates due to its mechanical performance, biocompatibility and biodegradability. Additionally, SF can regulate the formation of HAP and form organic-inorganic nanohybrid structures. Therefore, this project aims at 3D bioprinting of biomineralized SF composite scaffolds to mimick natural bone tissue.
Bone injuries and defects are a serious and costly health issue, and bone tissue engineering is one possible approach that aims at restoring the function of these damaged bone tissues with functional grafts. The next generation of engineering bone tissue aims at mimicking physiological bone tissue component and function by generating more complex and structurally organized implants. Natural bone is a living and complex tissue with various types of cells and nanohybrid organic-inorganic components. Bone cells (including osteoblasts, osteoclasts, osteocytes and mesenchymal stem cells) have a remarkable capacity for bone growth, regeneration, and remodeling. Bone extracellular matrix (ECM) is formed by a series of complex events involving mineralization with calcium phosphate in the form of hydroxyapatite (HAP) on ECM proteins primarily consisting of collagen type I. In order to mimic the natural bone tissue, two serious obstacles should be overcome. One is how to fabricate biomimetic bone scaffolds with living cells and maintain cell functions; the other one is how to mimic natural ECM with nanohybrid organic-inorganic components. Recent advances in 3D bioprinting provide a promising approach to produce cell-laden biomimetic scaffolds. 3D bioprinting allows precise placement of cells, biochemical factors and biomaterial in a layer-by-layer process with personalized implant design. For mimicking the natural ECM, silk fibroin (SF) is one of the ideal candidates due to its mechanical performance, biocompatibility and biodegradability. Additionally, SF can regulate the formation of HAP and form organic-inorganic nanohybrid structures. Therefore, this project aims at 3D bioprinting of biomineralized SF composite scaffolds to mimick natural bone tissue.
The aim of this project is try to fabricated cell-laden biomineralized silk fibroin biomimetic scaffold using 3D bioprinting technique. It will include two small aims, the first one is how to print stable silk fibroin biomineralized scaffold. The next one is how to print cell-laden scaffold.
The aim of this project is try to fabricated cell-laden biomineralized silk fibroin biomimetic scaffold using 3D bioprinting technique. It will include two small aims, the first one is how to print stable silk fibroin biomineralized scaffold. The next one is how to print cell-laden scaffold.
Jianhua Zhang, Jianhua.zhang@hest.ethz.ch, Institute for Biomechanics, ETH Zürich, Professorship Ralph Müller
Jianhua Zhang, Jianhua.zhang@hest.ethz.ch, Institute for Biomechanics, ETH Zürich, Professorship Ralph Müller