Register now After registration you will be able to apply for this opportunity online.
This opportunity is not published. No applications will be accepted.
Shape it up: controlled cell morphology relation to mechanical properties
In our group we experimentally characterize the mechanical properties of cells. In the present project we plan to develop and optimize microstructure fabrication techniques to drive cell attachment to well defined patterns to achieve controlled cell shape.
Keywords: Micro patterning, driven cell attachment and geometry, stiffness tunable substrate, traction force characterization
The project is composed of theoretical, experimental and computational work:
**Theoretical work**
- Gathering information from the literature the state of the art of micro and nanostructure fabrication techniques used to study cell rheology in general, and cytoskeleton structure and properties, in particular.
**Experimental work:**
- Establishing and testing the protocols of surface patterning techniques.
- Selection and optimization of the patterning techniques to be used in cell experiments.
- Characterization of the methods via complementary analytical techniques including fluorescence microscopy, atomic force microscopy and scanning electron microscopy
**Computational work:**
- Interpretation and evaluation of the acquired different experimental results using intensity based image processing techniques
The project is composed of theoretical, experimental and computational work:
**Theoretical work**
- Gathering information from the literature the state of the art of micro and nanostructure fabrication techniques used to study cell rheology in general, and cytoskeleton structure and properties, in particular.
**Experimental work:**
- Establishing and testing the protocols of surface patterning techniques.
- Selection and optimization of the patterning techniques to be used in cell experiments.
- Characterization of the methods via complementary analytical techniques including fluorescence microscopy, atomic force microscopy and scanning electron microscopy
**Computational work:**
- Interpretation and evaluation of the acquired different experimental results using intensity based image processing techniques
**SO WHAT CAN YOU LEARN?**
- Different imaging technique: fluorescent microscopy, atomic force microscopy and scanning electron microscopy.
- Special knowledge about cell culture and wet lab techniques.
- Image and data analysis methods
**SO WHAT CAN YOU LEARN?** - Different imaging technique: fluorescent microscopy, atomic force microscopy and scanning electron microscopy. - Special knowledge about cell culture and wet lab techniques. - Image and data analysis methods
Aron Horvath aron.horvath@hest.ethz.ch, Institute for Biomechanics, ETH Zürich, Professorship Jess Snedeker
Aron Horvath aron.horvath@hest.ethz.ch, Institute for Biomechanics, ETH Zürich, Professorship Jess Snedeker