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Detecting off-target effects of fluorescent reporter mouse models
Fluorescently labeled bone cells used for investigating bone mechanomics can be generated via CRISPR/Cas9 technology. However, off-target mutations need to be detected to provide solid genotype-phenotype correlations
Gene modifications in animal models can be generated by CRISPR/Cas9 technology as it is easier, faster, and more efficient than traditional methods. Thus, we employed the CRISPR/Cas9 genome editing technology to generate dual-fluorescent mouse models to investigate bone biomechanics. However, the off-target activity, which causes mutations at the sites other than the intended loci, is a major concern. To validate the functionality of fluorescent mouse models, this project aims to assess unwanted errors in the mouse genome via PCR. In future projects, the dual-fluorescent mice will allow us to rapidly identify osteoblasts and osteoclasts for in vivo experiments (tail loading, femur defect loading during bone adaptation and regeneration.
Gene modifications in animal models can be generated by CRISPR/Cas9 technology as it is easier, faster, and more efficient than traditional methods. Thus, we employed the CRISPR/Cas9 genome editing technology to generate dual-fluorescent mouse models to investigate bone biomechanics. However, the off-target activity, which causes mutations at the sites other than the intended loci, is a major concern. To validate the functionality of fluorescent mouse models, this project aims to assess unwanted errors in the mouse genome via PCR. In future projects, the dual-fluorescent mice will allow us to rapidly identify osteoblasts and osteoclasts for in vivo experiments (tail loading, femur defect loading during bone adaptation and regeneration.
The aim of the project is to assess the off-target effects of fluorescent reporter mouse models via PCR and designated tools for CRISPR
The aim of the project is to assess the off-target effects of fluorescent reporter mouse models via PCR and designated tools for CRISPR
Dilara Yilmaz (dilara.yilmaz@hest.ethz.ch)
Institute for Biomechanics, ETH Zurich, Professorship Ralph Müller
Dilara Yilmaz (dilara.yilmaz@hest.ethz.ch) Institute for Biomechanics, ETH Zurich, Professorship Ralph Müller