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Biomimetic osteocyte culture in 3D printed structures
Osteocytes are widely accepted to be the main mechanosensor in bone. Using micro 3D printing, advanced surface coating technology and live cell microscopy, we are establishing a novel 3D cell model to study the mechanotransduction mechanisms at play.
Keywords: osteocytes, 3D printing, bone biology, cell biology, biocompatibility, biomaterials, mechanobiology
Osteocytes form a cellular network embedded deeply in the bone matrix by connecting to each other and to other bone cells via dendritic processes passing through narrow channels. Fluid flow through this cavity and channel system seems to be one important factor for mechanotransduction by osteocytes. To study cellular behavior in a lifelike environment_ in vitro_, we are recreating network and channel systems similar to the lacuno-canalicular network using micro 3D printing based on Two Photon Polymerization. Surface coatings are applied to the printed structures in order to promote osteocyte adhesion. An osteocytic cell line as well as primary cells will be used in the model. Live cell microscopic analysis of cellular dynamics and function can be performed.
Osteocytes form a cellular network embedded deeply in the bone matrix by connecting to each other and to other bone cells via dendritic processes passing through narrow channels. Fluid flow through this cavity and channel system seems to be one important factor for mechanotransduction by osteocytes. To study cellular behavior in a lifelike environment_ in vitro_, we are recreating network and channel systems similar to the lacuno-canalicular network using micro 3D printing based on Two Photon Polymerization. Surface coatings are applied to the printed structures in order to promote osteocyte adhesion. An osteocytic cell line as well as primary cells will be used in the model. Live cell microscopic analysis of cellular dynamics and function can be performed.
A range of interesting projects is possible in the framework of the research described above, projects can be tailored to the interests and experience of the student. Specifically, students with experience in cell culture, simulation or surface functionalization are encouraged to apply. Please contact the publisher of this document if you would be interested in starting a project in this exciting field of research.
A range of interesting projects is possible in the framework of the research described above, projects can be tailored to the interests and experience of the student. Specifically, students with experience in cell culture, simulation or surface functionalization are encouraged to apply. Please contact the publisher of this document if you would be interested in starting a project in this exciting field of research.
Felicitas Flohr, flohrf@ethz.ch, Institute for Biomechanics, ETH Zürich, Professorship Ralph Müller
Felicitas Flohr, flohrf@ethz.ch, Institute for Biomechanics, ETH Zürich, Professorship Ralph Müller
Each year the IDEA League offers the students of its partner universities over 180 monthly grants for a short-term research exchange. In general, these grants are awarded based on academic merit. For more information visit http://idealeague.org/student-grant/