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Comparative biochemical and proteomic analysis of healthy and diseased human tendons
The objectives of this project strive to develop the experimental workflow protocol to analyze the matrix composition of healthy and diseased human tendons. This includes samples preparation, immunostaining, separation and identification of proteins using different mass spectroscopy techniques.
The precise mechanisms that make tendons more prone to injury as we get older are not well established. However, the altered matrix turnover is thought to play a key role.
Recently, characterization of tendon proteome started to gain momentum as a powerful tool to shed light into how alterations in the tendon extracellular composition contribute to (or result from) impaired repair of tendons in old individuals, and may help to better understand why the risk of injury increases with ageing.(1)
The objectives of this project strive to develop the experimental workflow protocol to analyze the matrix composition of healthy and diseased human tendons. This includes samples preparation, separation and identification of protein using different mass spectroscopy platforms (with the support of the Functional Genomics Centre Zurich).
The ultimate goal is to map the proteomic profile of human tendons in health and disease, and to use this knowledge to inform the design of the 3D tissue-engineered models that are currently developed in our laboratory.
Details of the project and emphasis on different aspects can be set according to the expertise and interest of the candidate.
The precise mechanisms that make tendons more prone to injury as we get older are not well established. However, the altered matrix turnover is thought to play a key role.
Recently, characterization of tendon proteome started to gain momentum as a powerful tool to shed light into how alterations in the tendon extracellular composition contribute to (or result from) impaired repair of tendons in old individuals, and may help to better understand why the risk of injury increases with ageing.(1)
The objectives of this project strive to develop the experimental workflow protocol to analyze the matrix composition of healthy and diseased human tendons. This includes samples preparation, separation and identification of protein using different mass spectroscopy platforms (with the support of the Functional Genomics Centre Zurich).
The ultimate goal is to map the proteomic profile of human tendons in health and disease, and to use this knowledge to inform the design of the 3D tissue-engineered models that are currently developed in our laboratory.
Details of the project and emphasis on different aspects can be set according to the expertise and interest of the candidate.
In general, the basic tasks are: 1. Literature review, particularly focusing on tendon and soft tissues proteomics (15%). 2. Protocol development and execution of experiments (50%). 3. Analyzing the data and producing figures (10%). 4. Writing the final report (Thesis) (25%).
Details of the project and emphasis on different aspects can be set according to the expertise and interest of the candidate.
We are looking for a motivated Masters student/intern with excellent communication and organization skills. The project demands a minimum of 20 hours of bench work per week, and would better suit students with a biology and/or bioinformatics background. Students with other but related background are also encouraged to apply.
External students (Erasmus, ETH Partner universities, ..etc) are welcome to apply, provided that they can secure their own funding for living expense
In general, the basic tasks are: 1. Literature review, particularly focusing on tendon and soft tissues proteomics (15%). 2. Protocol development and execution of experiments (50%). 3. Analyzing the data and producing figures (10%). 4. Writing the final report (Thesis) (25%).
Details of the project and emphasis on different aspects can be set according to the expertise and interest of the candidate.
We are looking for a motivated Masters student/intern with excellent communication and organization skills. The project demands a minimum of 20 hours of bench work per week, and would better suit students with a biology and/or bioinformatics background. Students with other but related background are also encouraged to apply.
External students (Erasmus, ETH Partner universities, ..etc) are welcome to apply, provided that they can secure their own funding for living expense
Contact: Amro Hussien, amro.hussien@hest.ethz.ch / Institute for Biomechanics, ETH Zürich / Professorship Jess Snedeker http://www.orthobiomech.ethz.ch/
Our laboratory is based in Balgrist Campus. Lengghalde 5, 8092 Zürich, Switzerland http://www.balgristcampus.ch/
Contact: Amro Hussien, amro.hussien@hest.ethz.ch / Institute for Biomechanics, ETH Zürich / Professorship Jess Snedeker http://www.orthobiomech.ethz.ch/
Our laboratory is based in Balgrist Campus. Lengghalde 5, 8092 Zürich, Switzerland http://www.balgristcampus.ch/