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Master thesis: Deimmunisation of a therapeutic protein
Therapeutic proteins have the advantage of high selectivity but are challenged with the risk of inducing an immune reaction in patients. Immunogenic proteins can induce the production of anti-drug-antibodies, resulting in altered pharmacokinetics, reduced efficacy and potentially severe anaphylactic or hypersensitivity reactions in patients.
Deimmunisation of a protein may be achieved by deletion of T cell epitopes through site directed mutagenesis.
Keywords: • Protein expression in E.coli
• Protein purification: His-tag affinity purification
• Site-directed mutagenesis / PCR
• Biophysical assays
• Enzymatic assays
• SDS-PAGE / Western blot
• R / Python: In silico immunogenicity prediction (optional)
• Neural-network based T cell epitope prediction tool (optional)
• Computational Biology / Structural Biology
• PyMol: Protein Stability, Protein-Protein interactions (optional)
In this project, the student gets the possibility to deimmunize a small protein inhibitor and
assess its inhibition capacity in collaboration with a postdoc and a PhD in pharmaceutical sciences. In addition, the student can learn bioinformatical tools to assess in silico immunogenicity of protein and directly validate the findings in vitro.
In this project, the student gets the possibility to deimmunize a small protein inhibitor and assess its inhibition capacity in collaboration with a postdoc and a PhD in pharmaceutical sciences. In addition, the student can learn bioinformatical tools to assess in silico immunogenicity of protein and directly validate the findings in vitro.
Remove potential T cell epitopes from a leech-derived serine protease inhibitor.
Remove potential T cell epitopes from a leech-derived serine protease inhibitor.
Rüthemann Peter, PhD
peter.ruethemann@unibas.ch
https://pharma.unibas.ch/de/personen/peter-ruethemann/
Please contact me for further information.