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High-throughput fluorescence microscopy with multifocal structured illumination
The space-bandwidth product is limited for conventional optical imaging systems. In this work, we want bypass this limit by employing multifocal structured illumination in conjunction with ultrafast beam scanning strategy.
High-throughput fluorescence microscopy with high imaging speed is always desired yet remains challenging due to the space-bandwidth product limit [1].
In our previous work, we proposed a rapid scanning large-field fluorescence microscopy method based on multifocal illumination and acousto-optic beam steering, achieving a large FOV (20 × 20 mm2), enhanced DOF (>1 mm), and real-time imaging performance [2,3]. In this project, we want to further push the limit to attain larger FOV (up to 50 x 50 mm2) and video-rate imaging speed while maintaining a high spatial resolution.
In the project, you will learn:
- How to design and build a customized fluorescence microscope
- Image processing skills with MATLAB
- Manuscript writing
Requirements:
- Strong motivation in scientific research
- Basic knowledge in optics
- Basic image processing skills with MATLAB
[1] Jongchan Park, David J. Brady, Guoan Zheng, Lei Tian, Liang Gao, "Review of bio-optical imaging systems with a high space-bandwidth product," Adv. Photon. 3(4) 044001 (26 June 2021) https://doi.org/10.1117/1.AP.3.4.044001
[2] Chen, Z., Mc Larney, B., Rebling, J., Deán‐Ben, X. L., Zhou, Q., Gottschalk, S., & Razansky, D. (2019). High-Speed Large-Field Multifocal Illumination Fluorescence Microscopy. Laser & Photonics Reviews, 1900070. https://doi.org/10.1002/lpor.201900070
[3] Chen, Z., Zhou, Q., Rebling, J., & Razansky, D. (2020). Cortex-wide microcirculation mapping with ultrafast large-field multifocal illumination microscopy. Journal of biophotonics, 13(11), e202000198. https://doi.org/10.1002/jbio.202000198
High-throughput fluorescence microscopy with high imaging speed is always desired yet remains challenging due to the space-bandwidth product limit [1]. In our previous work, we proposed a rapid scanning large-field fluorescence microscopy method based on multifocal illumination and acousto-optic beam steering, achieving a large FOV (20 × 20 mm2), enhanced DOF (>1 mm), and real-time imaging performance [2,3]. In this project, we want to further push the limit to attain larger FOV (up to 50 x 50 mm2) and video-rate imaging speed while maintaining a high spatial resolution.
In the project, you will learn: - How to design and build a customized fluorescence microscope - Image processing skills with MATLAB - Manuscript writing Requirements: - Strong motivation in scientific research - Basic knowledge in optics - Basic image processing skills with MATLAB
[1] Jongchan Park, David J. Brady, Guoan Zheng, Lei Tian, Liang Gao, "Review of bio-optical imaging systems with a high space-bandwidth product," Adv. Photon. 3(4) 044001 (26 June 2021) https://doi.org/10.1117/1.AP.3.4.044001 [2] Chen, Z., Mc Larney, B., Rebling, J., Deán‐Ben, X. L., Zhou, Q., Gottschalk, S., & Razansky, D. (2019). High-Speed Large-Field Multifocal Illumination Fluorescence Microscopy. Laser & Photonics Reviews, 1900070. https://doi.org/10.1002/lpor.201900070 [3] Chen, Z., Zhou, Q., Rebling, J., & Razansky, D. (2020). Cortex-wide microcirculation mapping with ultrafast large-field multifocal illumination microscopy. Journal of biophotonics, 13(11), e202000198. https://doi.org/10.1002/jbio.202000198
To Build a customized fluorescence microscopy system to achieve centimeter scale FOV, micron level spatial resolution and video-rate imaging speed.
To Build a customized fluorescence microscopy system to achieve centimeter scale FOV, micron level spatial resolution and video-rate imaging speed.
Dr. Zhenyue Chen (zhenchen@ethz.ch)
Tel: +41 44 6355931
http://www.razanskylab.org/
Co-supervised by:
Prof. Daniel Razansky, daniel.razansky@uzh.ch
Dr. Zhenyue Chen (zhenchen@ethz.ch) Tel: +41 44 6355931 http://www.razanskylab.org/
Co-supervised by: Prof. Daniel Razansky, daniel.razansky@uzh.ch