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Surface-immobilization of enzymes using mussel-inspired approaches and its application for surface-mediated hydrogel formation
Enzyme-immobilized surfaces can provide a platform for in situ crosslinking of hydrogels. Several enzyme-mediated hydrogel systems will be explored throughout the project. The immobilization methods will be optimized considering enzyme activities and efficiencies of hydrogel formation.
Keywords: hydrogel, enzyme immobilization, in situ crosslinking, tissue engineering
Enzyme-mediated crosslinking is one of the promising approaches for various hydrogel formation in tissue engineering researches. In the current project, several known enzyme-mediated crosslinking systems will be explored to demonstrate surface-mediated hydrogel formation. Mussel-inspired polydopamine coating will be exploited for immobilization of enzymes on the surface. Efficiencies of enzyme immobilization and activities of immobilized enzymes will be analyzed. Finally, enzyme-immobilized surfaces would be tested for in situ crosslinking of hydrogels, and rheological properties of prepared hydrogels would be analyzed. The established system could be further studied for bioprinting and injectable hydrogel applications.
Enzyme-mediated crosslinking is one of the promising approaches for various hydrogel formation in tissue engineering researches. In the current project, several known enzyme-mediated crosslinking systems will be explored to demonstrate surface-mediated hydrogel formation. Mussel-inspired polydopamine coating will be exploited for immobilization of enzymes on the surface. Efficiencies of enzyme immobilization and activities of immobilized enzymes will be analyzed. Finally, enzyme-immobilized surfaces would be tested for in situ crosslinking of hydrogels, and rheological properties of prepared hydrogels would be analyzed. The established system could be further studied for bioprinting and injectable hydrogel applications.
This project is aimed to develop a platform for surface-mediated hydrogel formation for tissue engineering applications.
This project is aimed to develop a platform for surface-mediated hydrogel formation for tissue engineering applications.