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Osseointegration of Bone Organoids on Orthopaedic Implants
Osseointegration is the direct structural and functional integration of a living bone at the implant surface. Here, we aims to 3D print bone organoids on medical grade titanium (Ti) implants, in order to develop an in vitro model of biomaterial osseointegration.
Keywords: Osseointegration, 3D printing, bone implants, hydrogel, organoids.
Bone regeneration and remodeling on the implant surface is very complex process which lasts for several weeks. Post-surgically osteoblast and other precursor cells migrate and adhere on the implant surface, this process would usually commence from day 1. Firm fixation and mechanical stability affect the clinical performance of bone implants.
The cells secrete non-collagenous proteins to simulate the cell adhesion and mineralization of the matrix. The process begins by the formation of a fragile afibrillar layer with a poorly formed osteoid. The matrix would be rich in calcium, phosphorous, non-collagenous proteins such as osteopontin and bone sialoprotein. This is followed by a deposition of thin calcified tissue by osteoblasts directly on the implant surface. The non-calcified matrix however is filled with precursor cells and blood vessels. This peri-implant calcification and bone formation contributes to primary implant fixation, this occurs about 2-weeks post-surgery.
Bone regeneration and remodeling on the implant surface is very complex process which lasts for several weeks. Post-surgically osteoblast and other precursor cells migrate and adhere on the implant surface, this process would usually commence from day 1. Firm fixation and mechanical stability affect the clinical performance of bone implants.
The cells secrete non-collagenous proteins to simulate the cell adhesion and mineralization of the matrix. The process begins by the formation of a fragile afibrillar layer with a poorly formed osteoid. The matrix would be rich in calcium, phosphorous, non-collagenous proteins such as osteopontin and bone sialoprotein. This is followed by a deposition of thin calcified tissue by osteoblasts directly on the implant surface. The non-calcified matrix however is filled with precursor cells and blood vessels. This peri-implant calcification and bone formation contributes to primary implant fixation, this occurs about 2-weeks post-surgery.
The goal is to establish an in vitro model to study bone/implant osseointegration. The student will develop collagen-based bone organoid on Ti surface. Intricate dynamics and maturation of bone organoid on implant surface will be investigated by advanced microscopy techniques and biochemical assays.
The goal is to establish an in vitro model to study bone/implant osseointegration. The student will develop collagen-based bone organoid on Ti surface. Intricate dynamics and maturation of bone organoid on implant surface will be investigated by advanced microscopy techniques and biochemical assays.
This project will be conducted at the Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore with Prof. Wilson Wang as the local host. Please contact Dr. Chris Steffi (doscs@nus.edu.sg) for further information. The project is only open to students registered at ETH Zurich with supervision through Institute for Biomechanics, ETH Zürich, Professorship Ralph Müller.
This project will be conducted at the Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore with Prof. Wilson Wang as the local host. Please contact Dr. Chris Steffi (doscs@nus.edu.sg) for further information. The project is only open to students registered at ETH Zurich with supervision through Institute for Biomechanics, ETH Zürich, Professorship Ralph Müller.