Functional Immune Repertoire AnalysisOpen OpportunitiesIn our lab we are analysing droplet-microfluidic generated multi-spectral images of single-cell secreting multiple protein simultaneously. As multifunctionalities of immune cells are understudied we are aiming at developing further data science tools for image analysis, cell detection, multi-functionality read-out and data visualization. This student project aims at building on previous data science and software development projects which have developed a comprehensive pipeline to analyse large fluorescent imaging files from single-cell secretion experiments inside droplets. The software includes droplet detection and tracking, cell identification and scoring, protein secretion read-out and classification, grouping and subpopulation data analysis together with plotting of visualisation tools. The image analysis includes Matlab, machine learning algorithms and Python Data Science tools. - Biomechanical Engineering, Diagnostic Applications, Modeling and Simulation
- Bachelor Thesis, Course Project, ETH Zurich (ETHZ), Internship, Semester Project
| This project uses droplet based microfluidics to encapsulate single-cells for antibody capture, characterization and B cell isolation. The current project involves cloning and expression of novel antigen into an antigen-library for antigen mapping at single-droplet resolution. The project is suited for Master projects and master thesis (>3 months) and involves, cloning, validation, expression analysis as well as single-droplet verification. Finally, antibody secretion experiment are planned to isolate single-antigen binders and B cell sequencing.
Current antigens revolve around the SARS Cov2 Nucleocapsid protein, RBD and envelope proteins to assess antibody cross-reactivity. - Biochemistry and Cell Biology, Biomedical Engineering, Genetic Immunology, Microbiology
- Bachelor Thesis, ETH Zurich (ETHZ), Internship, Semester Project
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